Fig. 1.

The outline of the procedure. (a) A schematic of a two-layered tissue with layer 1 thickness $z_1$. The input energy required to initiate ablation, $E_{\mathrm{th},\mathrm{surf}}$, must be found for at least four ablation depths, $z_{\mathrm{ab}}$, to calculate the unknown scattering lengths, ${\ell }_{\mathrm{s}}$, and ablation fluence thresholds, $F_{\mathrm{th}}$. (b) The $E_{\mathrm{th},\mathrm{surf}}$ at each depth is found by ablating tissue over a range of input pulse energies, measuring the percentage of the FoV that is ablated, and fitting Eq. (5) to find the 50% ablation point. (c) The measured $E_{\mathrm{th},\mathrm{surf}}$ values are fit to Eq. (4), from which ${\ell }_{\mathrm{s}}$ and $F_{\mathrm{th}}$ are extracted.