Fig. 3.

Scattering length and ablation threshold measurement results for porcine vocal fold sample #1. (a) TPAF images at $70\text{-}\mu \mathrm{m}$ depth before and after ablation, with processed images used to calculate the percentage of the FoV ablated. $\text{Scale bar}=50\mu \mathrm{m}$. (b) Ablation percentage data obtained from (a) fit to Eq. (5) to find the values of $E_{\mathrm{th},\mathrm{surf}}$ at the 50% ablation point. The shaded area corresponds to the 95% confidence interval of the fitted curve. (c) The $E_{\mathrm{th},\mathrm{surf}}$ at various ablation depths is fit to Eq. (4) to find $F_{\mathrm{th}}$ and ${\ell }_{\mathrm{s}}$ for the two layers of tissue, as summarized in Table 1. The epithelium transitions to the SLP at $62.0\pm 1.3\mu \mathrm{m}$ depth, as shown by the discontinuity. Uncertainty bars are 95% confidence interval values for $E_{\mathrm{th},\mathrm{surf}}$. The shaded area corresponds to 95% prediction intervals for $E_{\mathrm{th},\mathrm{surf}}(z_{\mathrm{ab}})$.