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. 2016 Nov 30;7:1907. doi: 10.3389/fmicb.2016.01907

Table 2.

Primers used to amplify the complete genome of lineage 5 NDV.

Fragment Location Forward primer sequence (5′ → 3′) Reverse primer sequence (5′ → 3′) Size
1 1–2792 ACCAAACAGAGAATCTGTGAGGTAC TCGGGCTACTGCTCGGAGAT 2792
2 2541–5926 GTCCAGCTACCTGCCGACTT GCCTGTCACGATGACTTGAGA 3386
3* 5673–9156 CTGAAGGCGCACTCACTACG AGATGTGCCGCCGTAGAAGAT 3484
4258–8657 CCTGCGGAGTGTGAAAGTCA GGAGCACTCCGGTTATCTTGG 4400
4 8644–12,183 AACCGGAGTGCTCCATCCC AGATTCCCAGCTGTGGGCAG 3540
5 11,745–15,192 CCCGGAACAGAAGCTGGTCA ACCAAACAGAGATTTGGTGAACGAC 3448
*

An additional primer was designed for fragment 3 which was located in the hypervariable region of the genome. Primer locations are based on NDV strain chicken/Banjarmasin/010/10 (HQ697254.1).