Fig. 3.

MHC-II sufficient pDC express IDO during EAE. (A) IDO mRNA expression in B cells, cDCs, pDCs and macrophages (MØ) sorted from dLNs of WT EAE mice 10 days after immunization. (B) EAE was induced in WT → WT and pIII + IV^−/− → WT chimeric mice, and IDO mRNA was measured in cDCs and pDCs sorted from dLN 10 days after immunization. (C) Mixed BM chimeric mice were generated by co-transferring (1:1) CD45.2 GFP⁺ WT and CD45.2 pIII + IV^−/− BM cells in lethally irradiated CD45.1 WT mice. WT GFP⁺ cDCs and pDCs, and pIII + IV^−/− cDCs and pDCs were sorted from dLNs 10 days after EAE induction based on CD45.2, CD11c, PDCA1 and GFP markers. IDO mRNA expression level in indicated cells (A–C) Results are representative of at least 3 independent experiments. Error bars depict mean ± SEM. One-way ANOVA with Bonferroni post Hoc test was used. *P < 0.05, ****P < 0.0001, NS = Non significant.