Subject area	Biology, Animal Science
More specific subject area	Cryoconservation of chicken semen
Type of data	Table
How data was acquired	Fluorescence microscopy, SCA (Sperm Class Analyzer)
Data format	Analyzed
Experimental factors	The natural osmoprotectant trehalose (0.1 M) was combined with different level (0–6%) of the permeant cryoprotectant dimethylacetamide to prevent cryodamages in chicken semen.
Experimental features	Sperm quality was assessed before and after freezing/thawing in chicken semen processed for cryopreservation using a range of quantitative dimethylacetamide/trehalose ratios to identify the most effective cryoprotective combination.
Data source location	Milano, Lodi (Italy)
Data accessibility	Data is available with this article