Figure 7.

Cytokine Expression Is Regulated By nuclear Ca²⁺ in a Cell-Specific Context

Data in (A)–(D) are from 16-HBE bronchial epithelial cells.

(A) RT-PCR shows IL-5 expression is increased by LTC₄ and this is prevented by cyclosporine A or after expression of the IP₃-NLS buffer. Data have been normalized to rest levels (% control).

(B) As in (A), but now thapsigargin is the stimulus.

(C) Western blot compares IL-5 protein expression following stimulation with LTC₄. IL-5 expression has been normalized to ERK levels.

(D) As in (C), but the stimulus is thapsigargin. Data in (E)–(H) are from RBL-1 mast cells.

(E) LTC₄ increases IL-5 transcription, and this is unaffected by IP₃-NLS but suppressed by cyclosporine A.

(F) As in (E), but thapsigargin is the stimulus.

(G) Western blot compares IL-5 protein expression following stimulation with LTC₄.

(H) As in (F), but thapsigargin is the stimulus.

In all panels, histograms summarize data from three independent experiments. In all histograms, data are represented as mean ± SEM.