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. 2016 Nov 17;64(4):790–802. doi: 10.1016/j.molcel.2016.10.009

Figure 5.

Figure 5

Structural Parameters of the 46 Metaphase Chromosomes Determined by 3D-CLEM

(A) 3D model of human chromosome 4, used to illustrate how chromosome geometry is obtained, for a diploid chromosome.

(B) A bar chart showing mean chromosome width for individual chromosomes 1–5 and 19–22. Error bars represent ±SEM.

(C) 2D scatterplot showing chromosome diameter versus length for chromosomes 1–5 and 19–22.

(D) A 2D scatterplot showing chromosome volume versus DNA content (http://www.ensembl.org) for the unambiguously characterized chromosomes. The calculated y value provides a standard curve formula to estimate chromosome condensation relative to DNA content, and vice versa. The volumes of the metaphase chromosomes have been adjusted to reflect chromosome volume with the periphery subtracted to improve the accuracy of our data (see Table S1 for raw values).

(E) 2D scatterplot showing the radial location within the cell of the mid-point for the chromosomes that could be unambiguously identified (numbers 1–5 and 19–22), relative to the center of the chromosome mass (red dot).