Figure 1. LV.GFP‐ and LV.hARSA‐injected NHP: treatment groups and tissue collection.

1. Coordinates for injection in normal NHP of the pilot group, study group 1 and study group 2, were calculated on the bases of pre‐surgery MRI scans.
2. 3D rendering of injected brains generated based on post‐surgery MRI scans showing the injection sites and the brain volume containing the vector suspension in the different treatments groups. EC, external capsule; Thal, thalamus; CR, corona radiata; ant, anterior; post, posterior.
3. The brain was cut in 6‐mm‐thick slices using an adjustable brain matrix, obtaining 9–10 slices/brain.
4. Each brain slice was divided along the midline and each part subdivided into blocks. The cervical spinal cord was cut in four blocks.