Figure 3.

Effect of makaluvamines over H₂O₂ toxicity and ROS release (A) Neuroprotective effect of compound 4 and 5 in the presence of 200 μM H₂O₂ after 12 h incubation; (B) Effect of Compound 5 co-incubation over ΔΨm measured by tetramethylrhodamine (TMRM) assay; (C) Compounds 2, 3, 4, and 5 at μM concentrations (0.05, 0.1 and 1 μM) inhibited ROS release versus H₂O₂ treated cells. The values are presented in percentage versus non-treated control cells, but the statistical comparison is made versus H₂O₂ treated cells.* p < 0.05 and ** p < 0.01. Data are mean ± SEM of three or more independent experiments performed intriplicate.