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. 2016 Oct 27;14(11):197. doi: 10.3390/md14110197

Figure 5.

Figure 5

Makaluvamine J (5) effect over SHSY5Y cells pretreated with H2O2. Vitamin E was added as a positive control. (A) Cytotoxicity of compound 5 over neuroblastoma cells; (B) Neuroprotective effect of compound 5 in the presence of 150 μM H2O2 after 6 h incubation; (C) Effect of compound 5 co-incubation on ΔΨm measured by TMRM assay; (D) Compound 5 inhibition of ROS release versus H2O2 treated cells; (E,F) Evaluation of GSH and catalase activity after 6 h incubation with 150 μM H2O2. GSH levels measured by ThiolTracker Violet (E) and catalase activity measured by Catalase Amplex Red kit (Invitrogen) (F). The values are presented in percentage versus non-treated control cells and compared to cells treated with 150 μM H2O2 alone.* p < 0.05 and ** p < 0.01.