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. 2016 Nov 30;6:38249. doi: 10.1038/srep38249

Figure 3.

Figure 3

(A,B) Expression of GFP-Arf6 rescues the effects of Rab14 KD on single lumen formation. Control and Rab14 KD cells were transfected with GFP-Arf6 and labeled for podocalyxin (red). Expression of GFP-Arf6 results in normal single lumen formation in Rab14 KD cells. (C,D) Expression of PtdIns-4-P5 kinase rescues Rab14 knockdown. Control and Rab14 KD cells were transfected with PtdIns-4-P5 kinase and labeled for podocalyxin. Expression of PtdIns-4-P5 kinase after Rab14 KD results in single AMIS formation. **p < 0.001. (E) The effects of GFP-Arf6 on single lumen formation are not mediated through Rac1 activation. Incubation of Rab14 KD cells with an inhibitor of Rac1 has no effect upon Arf6-mediated rescue. **p < 0.001, NS, not significant. (F) Wortmannin treatment rescues the Rab14 KD multi-AMIS phenotype. *p < 0.05, NS, not significant. Scale bars for (A,C), 10 μm. DAPI, nuclei (blue).