Figure 2. The expression of HUWE1 in mice preimplantation embryos after Huwe1 SiRNA microinjection.
The negative control refers to the embryos injected with the control scrambled RNA. (A) RT-PCR analysis of Huwe1mRNA expression in mice embryos obtained 48 h after microinjection. (B) Immunofluorescence of HUWE1 and H3K9 in mice preimplantation embryos obtained 72 h after microinjection. DAPI was used to stain the nucleus. The pictures were captured under the same parameters of staining and confocal setting.