Table 1. RNAi-related genes identified in the genome of ACP and their Accession IDs.
Group | RNAi-related* gene | Present in the genome | Accession ID | Querya cover | E-valueb | Identityc |
---|---|---|---|---|---|---|
RNAi core machinery: siRNA pathway | Dicer-2 | Yes | NW_007379804.1 | 67% | 8e-25 | 38% |
R2D2 | No | / | / | / | / | |
Ago-2 | Yes | NW_007379505.1 | 99% | 2e-86 | 56% | |
RNAi core machinery: miRNA pathway | Drosha | Yes | NW_007377741.1 | 77% | 0.0 | 64% |
DGCR8 | Yes | NW_007377449.1 | 77% | 1e-141 | 46% | |
Dicer-1 | Yes | NW_007377676.1 | 79% | 0.0 | 65% | |
Loquacious | Yes | NW_007377608.1 | 96% | 2e-71 | 43% | |
Loquacious-like | Yes | NW_007377495.1 | 84% | 1e-73 | 43% | |
Ago-1 | Yes | NW_007377764.1 | 96% | 0.0 | 86% | |
RNAi core machinery: piRNA pathway | Piwi/Aubergine | Yes | NW_007378348.1 | 92% | 2e-21 | 38% |
Ago-3 | Yes | NW_007377777.1 | 94% | 0.0 | 41% | |
Auxiliary factors (RISC) | FXMR | Yes | NW_007377542.1 | 54% | 2e-80 | 49% |
RNA helicase DDX | Yes | NW_007377487.1 | 89% | 0.0 | 76% | |
Staufen-RA | Yes | NW_007378277.1 | 18% | 5e-41 | 67% | |
Staufen-RB | Yes | NW_007378277.1 | 63% | 6e-17 | 44% | |
Maelstrom | Yes | NW_007378195.1 | 74% | 1e-13 | 24% | |
PRMT5 | Yes | NW_007377723.1 | 99% | 1e-44 | 40% | |
Clp-1 | Yes | NW_007378998.1 | 95% | 4e-177 | 58% | |
DsRNA uptake | Sid-1 | Yes | NW_007377471.1 | 99% | 0.0 | 46% |
Antiviral RNAi | Ars2-RA | Yes | NW_007377554.1 | 78% | 5e-151 | 55% |
Ars2-RB | Yes | NW_007377554.1 | 42% | 5e-30 | 48% | |
Egghead | Yes | NW_007378275.1 | 85% | 7e-149 | 70% | |
Nuclease | Nibbler (mut-7) | Yes | NW_007378502.1 | 55% | 2e-23 | 34% |
*See Table 2 for cited references for all of the RNAi-related genes used in this study.
a,b,cBased on protein-protein BLAST with query sequences from T. Castaneum (Coleoptera) against D. citri (Hemiptera).