Table 3. Primers used for dsRNA production and RT-qPCR.
Name | Sequence$ | Position* |
---|---|---|
dsAK-F | TAATACGACTCACTATAGGGAGTGGCATTCTTGTATGGCGTA | 57 |
dsAK-R | TAATACGACTCACTATAGGGAGGCCTGCAAGAATCTGTCTCC | 730 |
dsPCDB1-F | TAATACGACTCACTATAGGGAGGTTCACTGGAGCTAGGAGTGG | 3 |
dsPCDB1-R | TAATACGACTCACTATAGGGAGATTGTATCCATGAATGAGGC | 410 |
dsSOD-F | TAATACGACTCACTATAGGGAGGCGACTCCGGCATTTATC | 69 |
dsSOD-R | TAATACGACTCACTATAGGGAGGAAGTGTTCTTTGTGGAATAGATAGG | 847 |
dsTOM-F | TAATACGACTCACTATAGGGAGTTTCGGTGATTTCAGGAACTG | 45 |
dsTOM-R | TAATACGACTCACTATAGGGAGCGAGGAATTTTTCAAGATGG | 446 |
dsVIT-F | TAATACGACTCACTATAGGGAGAGAACAGATCCCAAGAACCC | 2241 |
dsVIT-R | TAATACGACTCACTATAGGGAGTTGTGATTTTTCAGCTGGGG | 2769 |
dsGFP-F | TAATACGACTCACTATAGGGAGCCAACACTTGTCACTACTTTCTCTT | 1 |
dsGFP-R | TAATACGACTCACTATAGGGAGGTAATGGTTGTCTGGTAAAAGGA | 480 |
AK_quant-F | CGGACTTGAGGGAGAACTGA | 611 |
AK_quant-R | GTGGTAGATACCGCGACCAG | 776 |
α-Tub-F | GCGTCTCTTCGGTTTGACGG | 896 |
α-Tub-R | CACTTCACCATCTGGTTGGC | 1092 |
$The underlined sequences correspond to the T7 sequence.
*The position of the primer is related to the gene.