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. 2016 Nov 30;6:38138. doi: 10.1038/srep38138

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(A) PA induced a concentration-dependent activation of ER stress in cell culture model. The cells were treated with the indicted concentrations of PA for 24 h and the key markers of ER stress were examined by western blotting. (B) Inhibitory effect of GCM on PA-mediated ER stress in cell culture. The cells were exposed to PA in the presence or absence of GCM for 24 h and then the changes of key ER stress markers were assessed by western blotting. (C) Inactivation of ER stress by GCM in liver samples analyzed by western blotting. (D) Inhibitory effect of GCM on GSK-3 activation of liver tissues induced by lipid metabolic disorder in MCD diet-mediated NASH model.