Table 1. IC₅₀ values for PhTX-343 inhibition of nAChR subunit combinations.

	VH (mV)	n	PhTX-343 IC50 (95% CI), μM
			Peak		Late
α4β2	−60	7	1.90	(1.01–3.60)	0.46	(0.28–0.75)
	−80	7	0.93	(0.41–2.09)	0.31	(0.18–0.54)
	−100	7	0.14	(0.09–0.22)	0.08	(0.049–0.132)
α4β2(V253F)	−60	14	0.62	(0.30–1.28)	0.063	(0.045–0.089)
	−80	10	0.75	(0.46–1.23)	0.12	(0.06–0.23)
	−100	8	0.11	(0.07–0.17)	0.014	(0.011–0.016)
α4β4	−60	9	0.97	(0.60–1.58)	0.17	(0.12–0.24)
	−80	9	0.35	(0.24–0.50)	0.065	(0.047–0.089)
	−100	6	0.29	(0.18–0.46)	0.027	(0.020–0.036)
α3β4	−60	7	0.29	(0.21–0.40)	0.088	(0.065–0.120)
	−80	8	0.080	(0.062–0.104)	0.012	(0.010–0.014)
	−100	9	0.077	(0.059–0.102)	0.0077	(0.0063–0.0093)
α3β4(F255V)	−60	7	0.31	(0.23–0.42)	0.074	(0.060–0.091)
	−80	9	0.038	(0.029–0.048)	0.012	(0.010–0.014)
	−100	8	0.017	(0.011–0.024)	0.005	(0.0036–0.0068)
α3β2	−60	7	64.5	(7.8–532)	1.59	(1.20–2.11)
	−80	12	9.26	(5.90–14.6)	1.37	(1.12–1.69)
	−100	8	3.59	(1.58–8.19)	0.37	(0.29–0.48)
α7	−60	6	6.71	(5.39–8.35)	6.56†	(5.30–8.12)
	−80	7	6.48	(5.12–8.21)	5.06†	(3.95–6.48)
	−100	9	8.80	(6.92–11.18)	6.77†	(5.44–8.42)
α1β1γδ	−60	8	27.1	(19.1–38.4)	18.0	(12.8–25.4)
	−80	8	15.1	(9.5–24.0)	11.9	(9.2–15.4)
	−100	9	3.26	(2.54–4.17)	2.44	(1.50–3.97)

IC₅₀ values for PhTX-343 inhibition of both peak and late current estimated from concentration-inhibition curves in Figs 2 and 4.

^†For α7 this is based on the area under the current trace as there was no stable late current.