Table 2. IC₅₀ values for PhTX-12 inhibition of nAChR subunit combinations.

	VH (mV)	n	PhTX-12 IC50 (95% CI), μM
			Peak		Late
α4β2	−60	8	3.87	(1.47–10.16)	0.23	(0.14–0.38)
	−80	9	2.17	(1.30–3.64)	0.28	(0.17–0.45)
	−100	11	1.64	(0.74–3.67)	0.044	(0.030–0.064)
α4β2(V253F)	−60	4	2.22	(1.44–3.42)	0.41	(0.28–0.60)
	−80	5	4.05	(2.22–7.36)	0.3	(0.25–0.35)
	−100	9	3.48	(1.40–8.70)	0.19	(0.13–0.27)
α4β4	−60	9	1.21	(0.70–2.07)	0.25	(0.16–0.40)
	−80	12	1.65	(0.96–2.83)	0.29	(0.21–0.40)
	−100	9	2.77	(1.30–5.92)	0.19	(0.14–0.27)
α3β4	−60	7	3.09	(2.13–4.47)	0.11	(0.08–0.14)
	−80	8	2.62	(1.71–4.01)	0.11	(0.08–0.15)
	−100	8	2.03	(1.22–3.36)	0.027	(0.022–0.033)
α3β4(F255V)	−60	5	1.63	(0.74–3.60)	0.52	(0.30–0.90)
	−80	5	2.78	(1.13–6.84)	0.36	(0.26–0.50)
	−100	5	1.05	(0.54–2.05)	0.068	(0.044–0.105)
α3β2	−60	9	65.2	(36.0–118.0)	3.20	(2.39–4.28)
	−80	12	60.6	(21.4–172)	2.57	(2.16–3.06)
	−100	10	74.4	(21.3–259.3)	1.38	(1.05–1.82)
α7	−60	12	18.7	(14.9–23.4)	8.32†	(6.55–10.58)
	−80	12	11.1	(7.2–17.1)	4.92†	(2.79–8.66)
	−100	10	12.1	(9.04–16.2)	5.02†	(3.01–8.40)
α1β1γδ	−60	9	3.16	(2.11–4.74)	0.070	(0.056–0.092)
	−80	11	6.18	(4.03–9.46)	0.10	(0.08–0.13)
	−100	8	5.02	(2.86–8.83)	0.071	(0.055–0.093)

IC₅₀ values for PhTX-12 inhibition of both peak and late current estimated from concentration-inhibition curves in Figs 2 and 4.

^†For α7 this is based on the area under the current trace as there was no stable late current.