Figure 1. Experimental protocol of SILAC labelling of organoids and mass spectroscopy analysis.

Outline of the SILAC mass spectrometry experimental design for organoids. Intestinal organoids from isolated crypts were grown in different SILAC media for 20 days. Different drugs or treatment can then be used and SILAC allows quantification of changes in protein expression. The effect of a class I HDAC inhibitor, CI994, was used to validate the feasibility of this approach.