Figure 7. Antibodies induced by vaccination with alum/4C-Staph formulation reduce CFU counts in the joints of infected animals.

(A,B) Antibody titration (Mean Fluorescent Intensity) against single combo antigens (A) and cytokine measurement (B) in knee joint washes of immunized mice 7 days after infection. Samples were collected from 9 to 10 animals/group from 2 independent experiments. Median and upper interquartile ranges are reported. (C) Log₁₀ CFU counts of knee joint washes collected from passively immunized mice 7 days p.i. Single dots represent single animals form 2 independent experiments, black lines report median values for each group and the grey dotted line shows the lower detectable value (ldv). For all the data reported above, the two-tailed Mann-Whitney U-test was used to assess significance.