Figure 1. Electrode tip localization in a rat brain using histology.

(a) Cresyl violet staining of a coronal histology section at the level of the target shows traces of the electrode trajectory (200 μm diameter) and its tip (dotted box, arrow); (b) Manual overlay of the histology section and the corresponding slice of the stereotaxic atlas⁸ with matching (continuous arrows) and non-matching (dotted arrows) regions indicated; (c) Zoomed-in version of (b), where the parafascicular nucleus (PF) that was targeted is highlighted (contour in red). The electrode tip landed outside the intended PF region.