Figure 3.

Relationships between myeloperoxidase anti‐neutrophil cytoplasmic antibody (MPO‐ANCA) affinity, MPO‐ANCA titre and neutrophil extracellular trap (NET) occurrences in glomerulus. (A) Correlation between MPO‐ANCA affinity and NET occurrences in the 30 subjects studied. The left panel indicated NET of citrullinated histone H3/MPO and the right panel PAD4/MPO. The Spearman's rank correlation coefficients for these were calculated to be r = −0.842, P < 0.001 and r = −0.818, P < 0.001, respectively, indicating, that ANCA affinity was significantly and strongly correlated with NET occurrence. (B) Correlation between MPO‐ANCA titre and NET occurrences. The left panel indicated NET of citrulinated histone H3/MPO and the right panel NET of PAD4/MPO. The Spearman's rank correlation coefficients for these were calculated to be r = 0.300, P = 0.054, r = 0.194, P = 0.152, respectively, indicating that correlation between MPO‐ANCA titre and NET occurrence was weak and not significant. (C) Correlation between MPO‐ANCA affinity (IC₅₀) and MPO‐ANCA titre. The Spearman's rank correlation coefficient was calculated to be r = −0.176, P = 0.176, indicating that correlation between MPO‐ANCA affinity and titre was weak and not significant.