Figure 2.

Immunocytochemical staining of rat type 1 MG with 9F5. A: Mixed glial cells (DIV10) were incubated with 9F5 (a) or anti‐Iba1 antibody (b). Fluorescence signals are shown individually (a, b) and after merging (c). B: Rat primary type 1 MG (a–d), rat type 2 MG (e–h), and peritoneal rat Mφ (i–l) were incubated with 9F5 or anti‐Iba1 antibody. Mouse IgG1 and rabbit IgG were used as negative controls (d, h, l). Fluorescence signals are shown individually and after merging. C: Rat type 1 MG were double‐stained with 9F5 (a) and anti‐lysosomal‐associated membrane protein‐1 (anti‐LAMP‐1) antibody (b). [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]