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. 2016 Jul 27;64(11):1938–1961. doi: 10.1002/glia.23034

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© 2016 The Authors. Glia Published by Wiley Periodicals, Inc.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Northern and WB analyses of rat Gpnmb mRNA and proteins. A: Total RNA (2.0 µg) from rat type 1 MG (lanes 1, 2), rat type 2 MG (lanes 3, 4), peritoneal rat Mφ (lane 5), LPS‐stimulated peritoneal rat Mφ (lane 6), thioglycolate‐elicited rat Mφ (lane 7), mouse MG5 MG (lane 8), adult rat brain (lane 9), and rat C6 glioma cells (lane 10) were subjected to blot analysis. The middle and bottom panels show Northern blot analysis of glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH) and ethidium bromide staining of 28S and 18S rRNA, respectively. B: WB analyses with 9F5 (a), anti‐GPNMB (b), and anti‐β‐actin antibodies (c) under DTT (−) conditions. Extracts (20 µg) were from mouse 6–3 MG (lane 1), mouse Ra2 MG (lane 2), rat type 1 MG (lane 3), LPS‐stimulated rat type 1 MG (lane 4), rat type 2 MG (lane 5), IL‐4‐stimulated rat type 2 MG (lane 6), rat neurons (lane 7), rat astrocytes (lane 8), mouse RAW 264.7 Mφ(lane 9), rat Mφ (lane 10), LPS‐stimulated rat Mφ (lane 11), thioglycolate‐elicited rat Mφ (lane 12), and rat neuroepithelial cells (lane 13).