Figure 9.

A furin siRNA inhibits the expression level of 9F5 antigen. HEK293 cells were cotransfected with pcDNA3.1‐Gpnmb/control small interfering RNA (siRNA) or pcDNA3.1‐Gpnmb/furin siRNA. A: At 72 hr after transfection, cells were double‐stained with 9F5 (red) and anti‐GPNMB (green) antibodies. B: The results shown in A were quantified and are given as means ± SEM (n = 3–4). Fluorescence intensities (9F5 and anti‐GPNMB) in cells transfected with pcDNA3.1‐Gpnmb/pcDNA3.1 were set at 100%. **P < 0.01 by Dunnett's multiple comparison test. C: Cell lysates were also subjected to immunoblot analysis for furin and β‐actin. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]