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. 2016 Jul 27;64(11):1938–1961. doi: 10.1002/glia.23034

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© 2016 The Authors. Glia Published by Wiley Periodicals, Inc.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Immunofluorescence analysis of cryostat sections of E14 rat eye. a–f: Coronal sections were probed with 9F5 and anti‐Iba1antibodies and then counterstained with Hoechst 33258 (a, b). Fluorescence signals are shown individually (a, b) and after merging (c). Arrows indicate 9F5 + Iba1− retinal cells, arrowheads, some 9F5 + Iba1+ cells. d–f: 9F5+ and Iba1+ cells are also shown at higher magnification. g–i:Coronal sections were probed with 9F5 and anti‐laminin antibodies and then counterstained with Hoechst 33258 (g, h). Fluorescence signals are shown individually and after merging. j–l:Coronal sections were probed with biotin‐conjugated 9F5 and anti‐MITF antibody. Fluorescence signals are shown individually and after merging. Section j–l demonstrated cell‐type‐specific expression of 9F5 antigen on the retinal pigment epithelium (arrow in c, f) and 9F5 + Iba1+ cells comprising a portion of the Iba1+ cells (arrowheads in c, f). [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]