Fig. 1.
HIF-1α stabilisation in ECFCs occurs rapidly at 1% O2. ECFCs were grown in 12% FCS-supplemented EBM-2 medium. a ECFCs were cultured at the indicated oxygen tension for 5 h. Protein lysates were probed using HIF-1α (top panel), with β-actin included as a loading control (bottom panel). b ECFCs were cultured in full EBM medium at 1% O2 for the indicated time range, starting at 5 min (5’) and ending at 240 min (240’). Cells were lysed and protein lysates were probed with HIF-1α (top panel), with β-actin included as a loading control (bottom panel) (n = 3). HIF-1α hypoxia inducible factor 1 alpha