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. 2016 Nov 29;7:174. doi: 10.1186/s13287-016-0434-9

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Human fetal and adult EPDCs express epicardial markers in culture. a Isolated fetal and adult EPDCs displayed a cobblestone appearance indicative of their epithelial phenotype (scale bar: 100 μm). b The presence of several epicardial markers was confirmed by PCR. Shown are two independently isolated fetal (F) and adult (A) EPDC cultures. Total adult myocardium, without epicardium, was used as negative control (-) and non-cultured epicardium as positive control (+). c Immunofluorescent staining showed that both EPDC populations were positive for the transcription factors WT1, TBX18 and TCF21 (scale bars: 100 μm). d ZO-1 depicted the tight junctions substantiating their epithelial phenotype (scale bar: 20 μm). EPDCs epicardial-derived cells