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. 2016 Nov 29;7:174. doi: 10.1186/s13287-016-0434-9

Fig. 2.

Fig. 2

Cultured EPDCs do not display markers of other heart-resident cell types. Immunofluorescent staining confirmed the absence of αSMA in both (a) fetal and (b) adult EPDCs, using mesenchymal cells as positive control (scale bar: 50 μm). c EPDCs were negative for the endothelial marker PECAM1 (scale bar: 50 μm). Human umbilical vein endothelial cells (HUVECs) were used as positive control. d qRT-PCR analysis confirmed the absence of markers attributed to cardiac progenitor cells, the endocardium, hematopoietic system, and myocardium. EPDCs epicardial-derived cells