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. 2016 Nov 29;7:174. doi: 10.1186/s13287-016-0434-9

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Fetal and adult EPDCs behave differently in tube formation assay. a Matrigel-based tube formation assay revealed that fetal EPDCs and sEPDCs failed to form tubes. In contrast, both adult EPDCs and sEPDCs were able to assemble in a network of tube-like structures. b Tubes expressed αSMA (scale bars: 100 μm). Tube formation is displayed as average length of tubes for (c) fetal and adult EPDCs and (d) their fully differentiated mesenchymal counterparts (sEPDCs). ^# p < 0.05. EPDCs epicardial-derived cells, sEPDCs spindle-shaped epicardial-derived cells