Molecular complementation of the drought-tolerant phenotype of hcf106 mutants. A, The phenotype of hcf106 and complementation plants at early seedling stage. Top, 7-d-old seedlings; bottom, immunoblot analysis of HCF106-3×FLAG; β-ACTIN was used as a loading control; bar = 1 cm. B, HCF106 expression level in Col-0, hcf106-1, hcf106-4, hcf106-1 complementation line, and hcf106-4 complementation line. Values are means ± sd (n = 3). C, Survival rate of the complementation plants after drought and rewatering treatment (n = 4 biological replicates, 16 plants of each replicate). Data represent means ± sd. D, Col-0, hcf106-1, hcf106-4, hcf106-1 complementation line, and hcf106-4 complementation line grown under normal growth conditions for 21 d, drought treated for 10 d, and then recovered for 5 d.