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. 2016 Oct 15;172(4):2491–2503. doi: 10.1104/pp.16.00889

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Figure 5. — HCF106 physically interacts with THF1. A, BiFC assay in Arabidopsis protoplasts showing the interaction between HCF106 and THF1. B, In vitro pull-down of MBP-tagged THF1 using 6×His-tagged HCF106 as determined by immunoblotting with anti-His antibody and anti-MBP antibody. C, Luciferase imaging of N. benthamiana leaf coinfiltrated with the Agrobacteria strains containing THF1-NLuc and/or CLuc-HCF106. D, THF1 protein levels in leaves of 4-week-old Col-0, hcf106-1, hcf106-4, hcf106-5, and thf1-1 determined with immunoblots. pTHF1, phosphorylated form of THF1; β-ACTIN was used as a loading control.