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. 2016 Oct 15;172(4):2491–2503. doi: 10.1104/pp.16.00889

Figure 6.

Figure 6.

Knockout of THF1 leads to H2O2 accumulation, enhanced stomatal aperture closure, reduced water loss, and increased tolerance to drought stress. A, Drought resistance assay. Col-0, hcf106-1, hcf106-4, and thf1-1 plants grown under normal growth condition for 21 d were treated with drought stress for 10 d, or 14 d, and then rewatered for 5 d. Survival rate of Col-0, hcf106-1, hcf106-4, and thf1-1 after drought treatment for 10 d (B) and 14 d (C; n = 4 biological replicates, 16 plants of each replicate). Stomatal density (D) and stomatal aperture (E) of the middle leaves of Col-0, hcf106-1, hcf106-4, and thf1-1 after drought stress for 7 d. F, Water loss in Col-0, hcf106-1, hcf106-4, and thf1-1 leaves (n = 3, each containing five fully expanded leaves from 4-week-old plants following drought stress for 7 d). G, Quantitative analysis of H2O2 levels in guard cells of Col-0, hcf106-1, hcf106-4, and thf1-1 (n = 3 leaves, 15 stomata per leaf leaves of 4-week-old plants following drought stress for 7 d). Data represent means ± sd. H, H2O2 accumulation in guard cells of Col-0, hcf106-1, hcf106-4, and thf1-1 labeled with CM-H2DCFDA probe.