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. 2016 Oct 17;172(4):2154–2164. doi: 10.1104/pp.16.00906

Figure 1.

Figure 1.

CFA-Ile exhibits the similar bioactivity and perception mechanism with COR. A, Chemical structure of (+)-7-iso-JA-l-Ile, COR, and CFA-Ile. CFA-Ile is constructed by the head part (red) of COR and the tail part (black) of (+)-7-iso-JA-l-Ile. B, VSP1 expression level in 7-d-old Col-0 seedlings treated with solvent (Mock), 100 µm JA-Ile, 1 µM CFA-Ile, or 1 µm COR, respectively, for 8 h. C, GUS staining of 7-d-old seedlings of AtVSP1::GUS transgenic plants treated with solvent (Mock), 100 μm JA-Ile, 0.1 μm CFA-Ile, or 0.1 μm COR, respectively, for 8 h. D, Restoration of the fertility of opr3 plants by CFA-Ile and COR. Pictures show the flower (top row), dehisced anther (second row), elongated filaments (third row), and main inflorescence (bottom row) of Ws (Wassilewskija) and opr3-treated with solvent (Mock), 400 µm CFA-Ile, or COR. E, Root length of 7-d-old Col-0 seedlings grown on MS with JA-Ile, CFA-Ile, or COR in different concentrations. Data are represented as mean ± sd (n = 30). F, CFA-Ile triggers the interaction of COI1 and JAZ1 in a dose-dependent manner. Recombinant JAZ1 and total plant extracts from the coi1-1 transgenic for HA-COI1 were used in the pull-down assay. HA-COI1 was detected with a HA antibody (top), and the input of JAZ1 was shown with Coomassie Blue staining (bottom). G, Activity comparison of JA-Ile, CFA-Ile, and COR on promoting the COI1-JAZ1 interaction in pull-down assay. HA-COI1 was detected with a HA antibody (top), and the input of JAZ1 was showed with Coomassie Blue staining.