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. 2016 Oct 17;172(4):2154–2164. doi: 10.1104/pp.16.00906

Figure 5.

Figure 5.

Molecular mechanism of active CFA conjugates in Arabidopsis. A, Phenotype of 10-d-old Col-0 and coi1-1 mutant seedlings grown on MS with solvent (Mock), 1 µm COR, or CFA conjugates. B, Quantitative analysis of root length of seedlings in A. Lowercase letters indicate statistical significant analysis. Parentheses are added to the lowercase letters for the statistical significant analysis of coi1-1. P < 0.05. Data are represented as mean ± sd (n = 30). C to G, Immunoblot of recovered Flag-COI1 by JAZ1 in pull-down assays in presence of 1 µm COR or CFA conjugates. COI1 was detected with a flag antibody (C–G, top), and the input of JAZ1 was showed with Coomassie Blue staining (C­–G, bottom). C, AtCOI1and AtJAZ1. D, SlCOI1 and SlJAZ1. E, OsCOI1a and OsJAZ1. F, OsCOI1b and OsJAZ1. G, OsCOI12 and OsJAZ1. H, ITC results of injecting CFA conjugates into AtCOI1 protein, respectively. Dissociation constant (Kd) of CFA-Ile, CFA-Leu, and CFA-Val are 2.315 µm, 7.874 µm, and 17.921 µm, respectively. As a control, CFA showed no change in heat capacity.