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. 2016 Oct 17;172(4):2154–2164. doi: 10.1104/pp.16.00906

Figure 6.

Figure 6.

Bioactivities of synthetic JA conjugates CFA-N-Leu and CFA-Ch-Gly. A, Chemical structures of CFA-Met, CFA-N-Leu, and CFA-Ch-Gly. B, Root length of 7-d-old Col-0 seedlings grown on MS with solvent (Mock), 10 µm CFA-Met, CFA-N-Leu, or CFA-Ch-Gly. Lowercase letters indicate statistical significant analysis; P < 0.05. Data are represented as mean ± sd (n = 30). C, GUS staining of 7-d-old seedlings of AtVSP1::GUS transgenic plants treated with solvent (Mock), 10 µm CFA-Met, CFA-N-Leu, or CFA-Ch-Gly for 8 h, respectively. D, Immunoblot of recovered HA-COI1 from crude protein extracts of 35S::HA-COI1 plants by JAZ1 in pull-down assays in presence of 1 µm CFA-Met, CFA-N-Leu, or CFA-Ch-Gly. HA-COI1 was detected with a HA antibody (top), and the input of was AtJAZ1 showed with Coomassie Blue staining (bottom).