Genetic analysis of WOX11/12 and WOX5/7. A and B, Leaf explants from 35Spro:WOX11 (A) and 35Spro:WOX11/wox5-1 wox7-1 (B) at 12 DAC on B5 medium. C, Ratio of adventitious root number per 15-DAC leaf explants from 35Spro:WOX11 and 35Spro:WOX11/wox5-1 wox7-1 on B5 medium. S, a small portion of leaf explants turned yellow and became senescence. Bars show sd from three biological repeats (n = 30 per repeat). *P < 0.05 and **P < 0.01 (two-sample t test, compared with 35Spro:WOX11). D, Leaf explants from 35Spro:WOX11-SRDX/pER8:WOX5 at 12 DAC cultured on B5 medium without (left) or with (right) 10 μm β-estradiol. None of the 29 leaf explants cultured on B5 medium without β-estradiol formed adventitious roots. Seven of 29 leaf explants cultured on B5 medium with 10 μm β-estradiol regenerated adventitious roots. Bars = 1 mm (A, B, and D).