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. 2016 Oct 26;172(4):2363–2373. doi: 10.1104/pp.16.01067

Figure 6.

Figure 6.

WOX11/12 directly regulate WOX5/7 expression. A, ChIP analysis showing enrichment of 3×FLAG-WOX11-GR and 3×FLAG-WOX12 in promoters of WOX5 and WOX7. Schematics of WOX5 and WOX7 gene structures are shown above ChIP data. Horizontal lines below genes show positions of PCR fragments in ChIP analysis. Bars show sd from three PCR repetitions. Results were confirmed with two independent biological repetitions. Values in Col-0 were arbitrarily fixed at 1.0. ACTIN (ACT) locus served as the negative control. *P < 0.05 and **P < 0.01 (two-sample t test, compared with Col-0 control). B and C, Relative ratio of firefly luciferase (LUC) to Renilla luciferase (REN) activity in tobacco leaves cotransformed with 35Spro:WOX11 or 35Spro:WOX12 and WOX5pro:LUC (B), and cotransformed with 35Spro:WOX11 or 35Spro:WOX12 and WOX7pro:LUC (C). Sole transformation with WOX5pro:LUC (B) or WOX7pro:LUC (C) served as the control. Bars show se from three biological repeats. Each biological repetition was performed with three experimental repetitions. *P < 0.05 and **P < 0.01 (two-sample t test, compared with control). D to F, GUS staining of 4-DAC leaf explants from WOX5pro:GUS (D) and two independent mWOX5pro:GUS lines (E and F) on B5 medium. Asterisks in E and F indicate dome-shaped root primordia. WOX5pro:GUS (D) served as the control. GUS signal could be occasionally observed in mesophyll in mWOX5pro:GUS (arrowheads in F). G, GUS staining of the primary root tip from mWOX5pro:GUS (line 1). Bars = 50 μm (D–G).