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. 2016 Oct 26;172(4):2363–2373. doi: 10.1104/pp.16.01067

Figure 7.

Figure 7.

WOX5/7 activation requires auxin. A, GUS staining of 35Spro:WOX11/WOX5pro:GUS in leaf explant at 4 DAC on B5 medium containing 1 μm NPA. For comparison, see Figure 4G. B, Quantitative analyses of WOX5-marked root primordia in 35Spro:WOX11/WOX5pro:GUS leaf explants at 4 DAC on B5 medium without or with 1 μm NPA. Bars show se from three biological repeats. Each repeat comprised 10 leaf explants. **P < 0.01 (two-sample t test). C, qRT-PCR analyses of transcript levels of WOX5 and WOX7 in 35Spro:WOX11 leaf explants at 4 DAC on B5 medium without (mock) or with 1 μm NPA. Bars show se from three biological repetitions. Each biological repetition was performed with three technical repetitions. *P < 0.05 and **P < 0.01 (two-sample t test, compared with mock). D, Leaf explants from pER8:WOX5 at 12 DAC on B5 medium containing 1 μm NPA and 10 μm β-estradiol. Note that leaf explants on medium containing NPA could not regenerate adventitious roots (Liu et al., 2014), but overexpression of WOX5 partially rescued this rooting defect. E, Statistical analysis of rooting rates shown in D. Bars show sd from three biological repeats (n = 30 per repeat). **P < 0.01 (two-sample t test). Bar = 500 μm (A) and 1 mm (D).