Cytoplasmic PEN3 preferentially colocalizes with trans-Golgi network markers. A and B, CLSM colocalization analyses in root epidermal cells of 5-d-old seedlings (A) coexpressing PEN3-GFP (P3-G, green) with VHA-a1-mRFP (Va1-R, magenta), and (B) with VTI12-mCherry/WAVE13R (Vti12-mCh, magenta). C, Quantitative colocalization analysis given as the percentage (%) of intracellular PEN3-GFP colabeled with marker compartments. Data are derived from CLSM images such as in A, B, and D with averages ± sd given for n = 15 cells per marker. D and E, Colocalization analysis of TGN marker SYP61-CFP (SYP61-C, blue), PEN3-GFP (green), and Golgi marker SYP32-mCherry/WAVE22R (S32-mCh, magenta) (D) after 37 min of 0.1% DMSO and (E) 37 min 50 μm
BFA. Bars = 10 μm.