Table II. Relative abundances of representative photosynthetic proteins and iron-sulfur cluster-containing proteins in the wild-type and nfu3 mutant plants.

Proteins were immunodetected as in Figure 5. Values (means ± se; n = 3) are given as the ratio to the protein levels in the wild type. Soluble proteins loaded on an equal total protein basis were used to determine the abundances of cFD, GRX, and FD-GOGATs. Thylakoid membrane proteins loaded on an equal fresh tissue weight basis were used to determine the levels of other proteins in this table. Symbols ++, ††, +++, and ++++ indicate that the protein binds classic 2Fe-2S clusters, Rieske-type 2Fe-2S clusters, 3Fe-4S clusters, and 4Fe-4S clusters, respectively. Asterisks indicate significant differences between the mutant and the wild type (Student’s t test: *, P < 0.05; **, P < 0.01; and ***, P < 0.001).

Parameter	Wild Type	nfu3-1	nfu3-2
PsaA++++	1.00 ± 0.16	0.13 ± 0.01*	0.12 ± 0.01*
PsaB++++	1.00 ± 0.05	0.08 ± 0.01**	0.13 ± 0.03***
PsaC++++	1.00 ± 0.08	0.14 ± 0.01*	0.11 ± 0.02*
LHCA2	1.00 ± 0.04	0.59 ± 0.02**	0.65 ± 0.01*
LHCA4	1.00 ± 0.08	0.87 ± 0.07	0.91 ± 0.03
FNR	1.00 ± 0.06	0.80 ± 0.02	0.78 ± 0.07
D1	1.00 ± 0.04	0.84 ± 0.08	0.78 ± 0.04*
CP43	1.00 ± 0.06	0.80 ± 0.01	0.69 ± 0.08*
CP47	1.00 ± 0.08	1.04 ± 0.17	1.05 ± 0.20
LHCB2	1.00 ± 0.09	0.80 ± 0.04	0.86 ± 0.09
LHCB4	1.00 ± 0.07	0.80 ± 0.09	0.94 ± 0.05
NdhB	1.00 ± 0.06	0.43 ± 0.01**	0.51 ± 0.06**
NdhH	1.00 ± 0.10	0.58 ± 0.03*	0.72 ± 0.03
AtpH	1.00 ± 0.06	0.79 ± 0.09	0.64 ± 0.02*
PetB	1.00 ± 0.05	1.01 ± 0.10	0.98 ± 0.10
PetC††	1.00 ± 0.08	1.13 ± 0.07	1.18 ± 0.06
cFD++	1.00 ± 0.06	1.82 ± 0.07***	1.68 ± 0.06**
GRX++	1.00 ± 0.27	2.27 ± 0.17*	2.74 ± 0.18**
FD-GOGATs+++	1.00 ± 0.11	0.54 ± 0.02*	0.55 ± 0.01*