Fig. 4.
Guinea pig cardiac ganglia express Kv4.2 transcripts, but inhibition of the voltage-dependent potassium current (IA) with 4-aminopyridine (4-AP) does not simulate PACAP-induced excitability. A: semiquantitative PCR demonstrating Kv4.2 transcript expression in brain, atrial muscle, and cardiac ganglia. NTC, no template control. B: averaged excitability curves for 10 cells prior to (□) and for 13 cells during (■) exposure to 1 mM 4-AP. Results demonstrate that 4-AP treatment to suppress KV4.2 currents had no effect on cardiac neuron excitability. APs, action potentials.