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A: genotyping for the cystathionine β-synthase (CBS) gene. CBS+/− and CBS+/−/C3H mice had 2 bands, at 450 and 308 bp; wild-type (WT) mice had 1 band, at 308 bp. B: restriction fragment length polymorphism-PCR for the Toll-like receptor 4 (TLR-4) gene. TLR-4 mutant (C3H and CBS+/−/C3H) mice had 2 bands, at 96 and 108 bp; nonmutant (WT and CBS+/−) mice had 1 band, at 204 bp.
