Increased migration of RPE cells in high glucose conditions. A: a scratch wound assay of mouse RPE cells cultured under different glucose conditions was used to determine their migration activity. Wound closure was monitored by phase microscopy, and digital images were captured at indicated time points. The degree of migration was assessed by determining the percent wound closure (**P < 0.01, ***P < 0.001, n = 3). B: migration of mouse RPE cells in different glucose conditions using a transwell assay. Please note a significant increase in the migration of RPE cells cultured in high glucose and osmotic stress conditions compared with normal glucose (**P < 0.01, n = 3). C: migration of human RPE cells incubated under different glucose conditions using a transwell assay. Please note high glucose and osmotic stress similarly resulted in a significant increase in the migration of human RPE cells (**P < 0.01, n = 3).