Skip to main content
. 2016 Jul 20;311(3):C418–C436. doi: 10.1152/ajpcell.00001.2016

Fig. 7.

Fig. 7.

Incubation of RPE cells with NAC restored their migration in high glucose conditions. A: expression of PEDF receptors in RPE cells cultured in different glucose conditions. RPE cells expressed both the PEDFR and laminin R receptor without any changes in their expression in different glucose condition. B: migration of PEDF−/− RPE cells in different glucose conditions using a transwell assay. Please note there is no significant difference in the migration of PEDF−/− RPE cells in high glucose conditions. C: migration of wild-type RPE cells incubated under various glucose conditions using a transwell assay. Please note a significant increase in migration of RPE cells in high glucose conditions that was restored to normal glucose level in the presence of NAC (***P < 0.001, n = 3). Unconditioned high glucose medium or normal glucose conditioned medium with NAC had no effect on migration of RPE cells. D: migration of wild-type and PEDF−/− RPE cells in response to high glucose with or without PDGF-BB. Please note PEDF−/− RPE cells are less migratory compared with wild-type cells regardless of the glucose conditions. In addition, PDGF-BB enhanced the migration of wild-type and PEDF−/− RPE cells regardless of glucose conditions. E and F: expression of PEDF in RPE cells in different glucose conditions. Please note a significant increase in the level of PEDF in RPE cells in high glucose that was reduced to near normal levels in the presence of NAC. (***P < 0.001, n = 3).