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Biotin deficiency impairs the activation of AMPK signaling pathway in DCs. phospho (p)AMPK and total AMPK levels were determined in biotin-deficient, control, and biotin-oversupplemented DCs before and after AICAR stimulation by ELISA. Bar graphs depict the levels of pAMPK (A) and AMPK (B) in DCs. Data are mean ± SE of 6 experiments.
