Table 1.
Primer sequences for real-time PCR studies
| Primer |
||||
|---|---|---|---|---|
| Gene | GenBank Accession No. | Forward (5′-3′) | Reverse (5′-3′) | Product Length, bp |
| Human Irx5 | NM_005853.4 | ccgccgccgccttctcctcgta | tggcgtcccttgtggcgttcttcc | 150 |
| Human β-actin | NM_001101.2 | gctcgtcgtcgacaacggct | caaacatgatctgggtcatcttctc | 353 |
| Rat Irx5 | NM_001030044.1 | cgccggcgcctcaagaaaga | gacccgcatcctccgccagac | 364 |
| Rat β-actin | NM_031144.1 | cctaaggccaaccgtgaaaagatg | gtcccggccagccaggtccag | 219 |
| Rat E2f1 | XM_230765.4 | agcgcctggcctatgtgacctg | tcgatggggccttgtttgctctta | 160 |
| Rat Pcna | NM_022381.2 | cacgtatatgccgggacctta | cagtggagtggcttttgtgaa | 230 |
| Rat Gadd45β | NM_001008321.1 | gaggcggccaaactgatgaatgtg | ggtctcgggcctcgtttgtgc | 226 |
| Rat Gadd45γ | NM_001077640.1 | acgagtccgccaaagtcctgaatg | gtctcccacgcgcacgatgtc | 161 |
| Rat p27kip1 | NM_031762.2 | gcggtgccttcaattgggtctca | cgggcttcttgggcgtctgctc | 232 |
Irx, Iroquois homeobox transcription factor; E2f1, E2F transcription factor 1; pcna, proliferating cell nuclear antigen; Gadd, growth arrest and DNA damage; p27kip1, cyclin-dependent kinase inhibitor 1B.