Fig. 3.

In vitro proliferative potential of conjunctival epithelial cells. A: calculation of the fold growth of conjunctival epithelial explants. B: comparison of fold growth of explants from different conjunctival epithelial locations. The bar chart displays the fold growth of cultures from the 6 conjunctival regions (**P < 0.01, ***P < 0.001). C: clonogenic capacity of cells isolated from different conjunctival origins. The bar chart shows the colony formation efficiency of cells isolated from the 6 conjunctival regions (**P < 0.01, ***P < 0.001). D: outgrowth of primary cultures from 6 conjunctival regions. Explant and outgrowth sizes were measured using ImageJ software (magnification: ×40). E and F: colony formation efficiency of cells isolated from different conjunctival regions with brightfield images of Rhodamine B-stained conjunctival colonies. Scale bars, 20 μm. The explant growth experiments in A, B, and D: n = 17 in 3 independent experiments; the colony formation efficiency experiment in C, E and F: n = 21 in 3 independent experiments. Sup, superior; Inf, inferior.