Fig. 6.

Tcf7l2 maintains conjunctival epithelial cell proliferation in vitro. After 7 days in culture, colonies were fixed and stained for Tcf7l2 (green) and p63α (red). Nuclei in the merged images were visualized with DAPI (blue). A prototypical holoclone is shown (n = 21 in 13 independent experiments). Tcf7l2 expression ubiquitously colocalized with p63α expression. Tcf7l2 presented stronger eccentric staining at further distances in the cytoplasm. Upon cultivation, Tcf7l2 translocated to the nucleus in cells near the peripheral clonal region, and the expression density of Tcf7l2 negatively correlated with that of p63α. The images illustrate the changes in Tcf7l2 and p63α expression. Tcf7l2 translocation is indicated by arrows. Scale bars, 20 μm.