Skip to main content
. 2016 Nov 30;3:16065. doi: 10.1038/mtm.2016.65

Figure 1.

Figure 1

RetroBMP4-green florescent protein (GFP) transduction and characterization of transduced muscle-derived cells (MDCs). Primary MDCs were isolated from the hind-limb skeletal muscles of three 3-week-old C57/BL10J mice using a modified preplate technique. The retroviral vectors encoding for BMP4 and the marker gene GFP (retroBMP4-GFP) were used for the transduction. (a) RetroBMP4-GFP transduction of MDCs. The efficiency of retro-BMP4-GFP transduction of all three MDC subpopulations was ~80% (48 hours after transduction, representative images). All populations were purified based on GFP signal by fluorescence-activated cell sorting (FACS) (After FACS, representative images). (b) BMP4 secretion levels of the transduced MDCs after purification by FACS. (n = 9, pooled data for three isolations, n = 3 for each isolation); (c) In vitro proliferation of BMP4-expressing MDCs. (n = 9, pooled data for three isolations, n = 3 for each isolation); (d) Cell survival of BMP4-expressing MDCs under oxidative stress. (n = 9, pooled data for three isolations, n = 3 for each isolation). Data are presented as mean ± SD.