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. 2016 Jun 3;26(7):775–786. doi: 10.1038/cr.2016.66

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Copyright © 2016 Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences

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MutSβ physically interacts with Polβ. (A) Gel shift assay to determine the interaction of MutSβ, Polβ and a ³²P-labeled primer extension substrate containing a (CAG)₅ hairpin. (B, C) Co-immunoprecipitation (Co-IP) assay to determine interactions between MutSβ and Polβ in HeLa nuclear extract. Co-IP by IgG was used as a negative control. (D) Far-western blot analysis showing direct interaction between purified MutSβ and Polβ. PCNA and polδ were used as positive and negative controls, respectively.